Rac Regulates Its Effector Phospholipase Cγ2 through Interaction with a Split Pleckstrin Homology Domain*S⃞

@article{Walliser2008RacRI,
  title={Rac Regulates Its Effector Phospholipase Cγ2 through
 Interaction with a Split Pleckstrin Homology
 Domain*S⃞},
  author={Claudia Walliser and Michael Retlich and Richard Harris and Katy L. Everett and Michelle B. Josephs and Petra Vatter and Diego Esposito and Paul C Driscoll and Matilda Katan and Peter Gierschik and Tom D. Bunney},
  journal={The Journal of Biological Chemistry},
  year={2008},
  volume={283},
  pages={30351 - 30362}
}
Several isoforms of phospholipase C (PLC) are regulated through interactions with Ras superfamily GTPases, including Rac proteins. Interestingly, of two closely related PLCgamma isoforms, only PLCgamma(2) has previously been shown to be activated by Rac. Here, we explore the molecular basis of this interaction as well as the structural properties of PLCgamma(2) required for activation. Based on reconstitution experiments with isolated PLCgamma variants and Rac2, we show that an unusual… CONTINUE READING