Rabies virus entry into cultured rat hippocampal neurons.

  title={Rabies virus entry into cultured rat hippocampal neurons.},
  author={Paul Wayne Lewis and Thomas L. Lentz},
  journal={Journal of neurocytology},
  volume={27 8},
Rabies virus entry into cultured hippocampal neurons was investigated by immunofluorescence and electron microscopy. Hippocampal neurons were susceptible to rabies virus infection and became filled with viral antigen 1 day after infection. Infection was inhibited by the lysosomotropic agents chloroquine and ammonium chloride. To study entry, neurons were adsorbed with rabies virus at 4 degrees C and warmed to 37 degrees C for short periods of time prior to fixation and localization of viral… CONTINUE READING
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