Rabbit tumor necrosis factor: mechanism of action.

  title={Rabbit tumor necrosis factor: mechanism of action.},
  author={Michael Ruff and George E. Gifford},
  journal={Infection and immunity},
  volume={31 1},
Rabbit tumor necrosis factor (TNF) was examined for effects on normal and transformed cells in culture. Several assays for killing of L-929 cell targets were developed, and their sensitivities were compared. Normal cells were not killed by TNF, and the discrimination between normal and transformed cells was shown not to be due to a cell cycle-dependent mechanism. TNF killing of L-929 cells was delayed for 10 to 12 h and thereafter showed concentration and time-dependent increases in cytolysis… CONTINUE READING