Rabbit skeletal muscle glycogen synthase expressed in COS cells. Identification of regulatory phosphorylation sites.

@article{Skurat1994RabbitSM,
  title={Rabbit skeletal muscle glycogen synthase expressed in COS cells. Identification of regulatory phosphorylation sites.},
  author={Alexander V. Skurat and Yun Hua Wang and Peter Roach},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 41},
  pages={25534-42}
}
Rabbit skeletal muscle glycogen synthase contains multiple sites for phosphorylation. To investigate the relative importance of these sites, the enzyme was overexpressed in COS M9 cells, and Ser-->Ala mutations were introduced singly, or in combinations, at nine known phosphorylation sites. Overexpressed wild-type enzyme had a very low +/- glucose-6-P activity ratio of approximately 0.01, indicative that the glycogen synthase is in a highly phosphorylated state. No single Ser-->Ala mutation was… CONTINUE READING

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