Rabbit placental relaxin: purification and immunohistochemical localization.

@article{Eldridge1985RabbitPR,
  title={Rabbit placental relaxin: purification and immunohistochemical localization.},
  author={Richard Eldridge and Phillip A Fields},
  journal={Endocrinology},
  year={1985},
  volume={117 6},
  pages={2512-9}
}
Rabbit placentas were extracted with 0.7 N HCl-acetone (3:5, vol/vol) containing protease inhibitors. Gel filtration (Sephadex G-50) followed by ion exchange chromatography (carboxymethyl cellulose) separated a bioactive relaxin-like fraction with a specific activity of 8.5 U/mg protein as determined by the in vitro mouse uterus bioassay. Column… CONTINUE READING