RT-qPCR-based quantification of small non-coding RNAs.

  title={RT-qPCR-based quantification of small non-coding RNAs.},
  author={Fjoralba Zeka and Pieter Mestdagh and Jo Vandesompele},
  journal={Methods in molecular biology},
MicroRNAs (miRNAs) are small non-coding RNA molecules that negatively regulate messenger RNA (mRNA) translation into protein. MiRNAs play a key role in gene expression regulation, and their involvement in disease biology is well documented. This has fueled the development of numerous tools for the quantification of miRNA expression levels. These tools are… CONTINUE READING