RT-PCR based analysis of cell culture negative stools samples from poliomyelitis suspected cases.

@article{Santos2002RTPCRBA,
  title={RT-PCR based analysis of cell culture negative stools samples from poliomyelitis suspected cases.},
  author={Ana Paula Pires dos Santos and Eliane Veiga da Costa and Silas S. Oliveira and Michele C Souza and Edson Elias da Silva},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2002},
  volume={23 3},
  pages={149-52}
}
BACKGROUND Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several serotypes of enterovirus which possess the potential to cause neurological disorders are not easily isolated in the cell culture systems used for the AFP diagnosis. OBJECTIVES Our goal was to look into the presence of enterovirus genomes in fecal suspensions previously considered negative by cell culture procedures, using RT-PCR. STUDY DESIGN One hundred and seventy… CONTINUE READING