RT-PCR assays for in vivo expression ofVibrio alginolyticus virulence genes in cultured giltheadDicentrarchus labrax andSparus aurata

Abstract

In this study, we investigated the pathogenic potency of twoVibrio alginolyticus strains isolated from cultured giltheadDicentrarchus labrax andSparus aurata, in Tunisia. The enzymatic activities of the extracellular products of the studied strains characterized with the API-ZYM system, revealed the existence of several activities. The 50% lethal doses levels were then determined, virulence levels ranged from 1.03×105 to 1.0×106 CFU/fish. In addition, we also searched for the presence of eightVibrio cholerae virulence genes:ctxA, zot, ace, toxR, toxS, toxRS, toxT, and Virulence Pathogenicity Island (VPI), in the genome of two isolated strains ofV. alginolitycus. Thein vivo expression oftoxR, toxS, VPI andace genes in both fish species, was tested by reverse transcriptase polymerase chain reaction. Indeed, we have noted that for each strain all the virulence genes selected were expressed in sea bream as well as in sea bass.

DOI: 10.1007/BF03175600

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Cite this paper

@article{Abdallah2009RTPCRAF, title={RT-PCR assays for in vivo expression ofVibrio alginolyticus virulence genes in cultured giltheadDicentrarchus labrax andSparus aurata}, author={Fethi Ben Abdallah and Kamel Chaieb and H{\'e}la Kallel and Amina Bakhrouf}, journal={Annals of Microbiology}, year={2009}, volume={59}, pages={63-67} }