RT-PCR/electrospray ionization mass spectrometry approach in detection and characterization of influenza viruses

@article{Deyde2011RTPCRelectrosprayIM,
  title={RT-PCR/electrospray ionization mass spectrometry approach in detection and characterization of influenza viruses},
  author={Varough M. Deyde and Rangarajan Sampath and Larisa V Gubareva},
  journal={Expert Review of Molecular Diagnostics},
  year={2011},
  volume={11},
  pages={41 - 52}
}
Reverse-transcription PCR (RT-PCR) coupled with electrospray ionization mass spectrometry (ESI-MS) is a high-throughput nucleic acid-based technology that relies on the accurate measurement of the molecular weight of PCR amplicons that can be used to deduce the base counts (number of As, Gs, Cs and Ts) of DNA. These amplicons represent highly variable regions with information-rich sequences, which are flanked by broad-range primers designed based on highly conserved loci. This technology was… 
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