RSF1010-based shuttle vectors for cloning and expression in Pasteurella multocida.

Abstract

The broad host-range cloning vectors, pJRD215 and pMMB67EH, were evaluated for stability and cloning efficiency in Pasteurella multocida. Transformation of P. multocida by electroporation was unreliable and poorly efficient regardless of whether the transforming DNA was isolated from E. coli or P. multocida. Both vectors contain a mob site that enabled… (More)

Topics

Cite this paper

@article{Lee1997RSF1010basedSV, title={RSF1010-based shuttle vectors for cloning and expression in Pasteurella multocida.}, author={M. D. Lee and Adam D. Henk}, journal={Veterinary microbiology}, year={1997}, volume={54 3-4}, pages={369-74} }