RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits.

@article{Jeronimo2004RPAP1AN,
  title={RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits.},
  author={C{\'e}lia Jeronimo and M F Langelier and Mahel Zeghouf and Marilena Cojocaru and Dominique Bergeron and Dania Baali and Diane Forget and Sanie Mnaimneh and Armaity P. Davierwala and Jeff Pootoolal and Mark Chandy and Veronica Canadien and Bryan K. Beattie and Dawn P. Richards and Jerry L. Workman and Timothy R. Hughes and Jack Greenblatt and Benoit Coulombe},
  journal={Molecular and cellular biology},
  year={2004},
  volume={24 16},
  pages={7043-58}
}
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the general transcription factors TFIIB and TFIIF, the RNAPII phosphatase Fcp1, and a novel 153-kDa polypeptide of unknown function that we named RNAPII-associated protein 1 (RPAP1). The TAP-tagged RNAPII… CONTINUE READING