RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases.

Abstract

Bloom (BLM) and Werner (WRN) syndrome proteins are members of the RecQ family of SF2 DNA helicases. In this paper, we show that restricting the rotational DNA backbone flexibility, by introducing vinylphosphonate internucleotide linkages in the translocating DNA strand, inhibits efficient duplex unwinding by these enzymes. The human single-stranded DNA… (More)

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@article{Garcia2004RPAAT, title={RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases.}, author={Patrick L. Garcia and Glyn Bradley and Christopher J Hayes and Sussie Lerche Krintel and Panos Soultanas and Pavel Janscak}, journal={Nucleic acids research}, year={2004}, volume={32 12}, pages={3771-8} }