RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride and Telmisartan in Bulk and in a Synthetic Mixture

  • Sattar Navaneetha, Medidi Srinivas, Sri Venkateshwara
  • Published 2014

Abstract

A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for simultaneous estimation of Metformin hydrochloride and Telmisartan in bulk drug and in a synthetic mixture. The analysis was carried out using Zodiac ODS C18 (4.6 x 250mm, 5μm, Make: Zodiac Life Sciences), pre-packed column. The separation was carried out using a mobile phase containing a buffer of pH 4.0, Acetonitrile and Methanol (30:30:40 v/v/v), was pumped at a flow rate of 1.4 mL/min with UV-detection at 228 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 2.523 minute for Metformin hydrochloride and 5.437 minute for Telmisartan. The method was validated and shown to be linear for Metformin hydrochloride and Telmisartan. The correlation coefficients for Metformin and Telmisartan are 0.996 and 0.998 respectively.

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Cite this paper

@inproceedings{Navaneetha2014RPHPLCMF, title={RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride and Telmisartan in Bulk and in a Synthetic Mixture}, author={Sattar Navaneetha and Medidi Srinivas and Sri Venkateshwara}, year={2014} }