ROS Production and NF-κB Activation Triggered by RAC1 Facilitate WNT-Driven Intestinal Stem Cell Proliferation and Colorectal Cancer Initiation

Abstract

The Adenomatous Polyposis Coli (APC) gene is mutated in the majority of colorectal cancers (CRCs). Loss of APC leads to constitutively active WNT signaling, hyperproliferation, and tumorigenesis. Identification of pathways that facilitate tumorigenesis after APC loss is important for therapeutic development. Here, we show that RAC1 is a critical mediator of tumorigenesis after APC loss. We find that RAC1 is required for expansion of the LGR5 intestinal stem cell (ISC) signature, progenitor hyperproliferation, and transformation. Mechanistically, RAC1-driven ROS and NF-κB signaling mediate these processes. Together, these data highlight that ROS production and NF-κB activation triggered by RAC1 are critical events in CRC initiation.

DOI: 10.1016/j.stem.2013.04.006

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@inproceedings{Myant2013ROSPA, title={ROS Production and NF-κB Activation Triggered by RAC1 Facilitate WNT-Driven Intestinal Stem Cell Proliferation and Colorectal Cancer Initiation}, author={Kevin B. Myant and Patrizia Cammareri and Ewan J. McGhee and Rachel A. Ridgway and David J. Huels and Julia B. Cordero and Sarah Schwitalla and Gabriela Kalna and Erinn-Lee Ogg and Dimitris Athineos and Paul Timpson and Marcos Vidal and Graeme I. Murray and Florian R. Greten and Kurt I. Anderson and Owen J. Sansom}, booktitle={Cell stem cell}, year={2013} }