RNase-sensitive glucocorticoid-receptor complexes from HELA cell nuclei.


Dexamethasone-receptor complexes can be extracted from HeLa cell nuclei by mild sonication. These complexes can account for 800-1000 binding sites/cell, and are indistinguishable from salt-extractable ones as judged by sucrose gradient centrifugation in the presence of 0.3 M KCl, showing a sedimentation coefficient of 3.6 S. These complexes, however, broadly sediment in the 7 to 3.6 S region of low salt sucrose gradients. Enzymatic treatment of soluble extracts from nuclear sonicates shows that RNA is associated to dexamethasone-receptor complexes.

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@article{Rossini1983RNasesensitiveGC, title={RNase-sensitive glucocorticoid-receptor complexes from HELA cell nuclei.}, author={Gian Paolo Rossini and Bruno Barbiroli}, journal={Biochemical and biophysical research communications}, year={1983}, volume={113 3}, pages={876-82} }