RNAi screening comes of age: improved techniques and complementary approaches

@article{Mohr2014RNAiSC,
  title={RNAi screening comes of age: improved techniques and complementary approaches},
  author={Stephanie E. Mohr and Jennifer A. Smith and Caroline E. Shamu and Ralph A. Neum{\"u}ller and Norbert Perrimon},
  journal={Nature Reviews Molecular Cell Biology},
  year={2014},
  volume={15},
  pages={591-600}
}
Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells and in vivo in model organisms. These screens have resulted in the identification of new cellular pathways and potential drug targets. Considerable progress has been made to improve the quality of RNAi screen data through the development of new experimental and bioinformatics approaches. The recent availability of genome-editing strategies, such as the CRISPR… CONTINUE READING