RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.

@article{PalBhadra2002RNAiRM,
  title={RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.},
  author={Manika Pal‐Bhadra and Utpal Bhadra and James A. Birchler},
  journal={Molecular cell},
  year={2002},
  volume={9 2},
  pages={
          315-27
        }
}
RNA silencing in Drosophila
A nuclear perspective on RNAi pathways in metazoans.
RNAi-mediated pathways in the nucleus
TLDR
RNA interference is an evolutionarily conserved mechanism that uses short antisense RNAs that are generated by 'dicing' dsRNA precursors to target corresponding mRNAs for cleavage to facilitate the directed silencing of specific genomic regions.
RNA Interference (RNAi) and MicroRNAs
TLDR
It is clear that a conserved biological response to double-stranded RNA-induced (dsRNA) gene silencing mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, such as transposons and RNA viruses, and regulates the expression of protein-coding genes.
RNAi-Mediated Targeting of Heterochromatin by the RITS Complex
TLDR
The purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast is described and a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochROMatin complexes and epigenetic genesilencing at specific chromosomal loci is suggested.
Silencing in Arabidopsis T-DNA Transformants: The Predominant Role of a Gene-Specific RNA Sensing Mechanism versus Position Effects
TLDR
It is proposed that the RNA sensing mechanism described is a genome surveillance system that eliminates RNA corresponding to excessively transcribed genes, including transgenes, and so plays an important role in genome defense.
Transcriptional silencing of a transgene by RNAi in the soma of C. elegans.
TLDR
This type of silencing can be distinguished from transgene silencing in the soma by its inability to be transmitted across generations and its dependence on the rde-1 gene, and is defined as RNAi-induced Transcriptional Gene Silencing (RNAi-TGS).
A correlation between Long noncoding RNA and unpaired DNA silencing in Drosophila
TLDR
It is suggested that functional RNA-Piwi complex might create a silencing driven chromatin configuration by accumulating histone modifying enzymes at the Adh-w promoter target, which represents a novel mechanism to repress new transposon and foreign DNA insertions for protection of genome integrity.
C. elegans RNA-dependent RNA polymerases rrf-1 and ego-1 silence Drosophila transgenes by differing mechanisms
Drosophila possesses the core gene silencing machinery but, like all insects, lacks the canonical RNA-dependent RNA polymerases (RdRps) that in C. elegans either trigger or enhance two major small
RNA-Interference Components Are Dispensable for Transcriptional Silencing of the Drosophila Bithorax-Complex
TLDR
It is concluded that the Dicer-2/Argonaute-2 RNAi pathway, despite its role in pairing sensitive gene silencing of transgenes, does not have a role in PcG dependentsilencing of major homeotic gene cluster loci in Drosophila.
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References

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Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase
TLDR
The cloning of qde-1, the first cellular component of the gene-silencing mechanism to be isolated, defines a new gene family conserved among different species including plants, animals and fungi, which is similar to an RNA-dependent RNA polymerase found in the tomato.
A species of small antisense RNA in posttranscriptional gene silencing in plants.
TLDR
The 25-nucleotide antisense RNA detected in transgene-induced PTGS is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
SDE3 encodes an RNA helicase required for post‐transcriptional gene silencing in Arabidopsis
TLDR
It is concluded that SDE3, like the previously described RNA polymerase encoded by SDE1, acts at a stage in the mechanism that is circumvented when PTGS is mediated by TRV.
RNA–DNA Interactions and DNA Methylation in Post-Transcriptional Gene Silencing
TLDR
An epigenetic model of PTGS in which transgene methylation is associated with an RNA–DNA interaction that ensures that PTGS is maintained is supported.
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