RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy

@article{Allen2004RNAimediatedRO,
  title={RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy},
  author={Robert S. Allen and Anthony G. Millgate and Julie A. Chitty and Jennifer Thisleton and James Arthur Miller and Anthony J. Fist and W. L. Gerlach and Philip J. Larkin},
  journal={Nature Biotechnology},
  year={2004},
  volume={22},
  pages={1559-1566}
}
We report on the silencing of codeinone reductase (COR) in the opium poppy, Papaver somniferum, using a chimeric hairpin RNA construct designed to silence all members of the multigene COR family through RNA interference (RNAi). After gene silencing, the precursor alkaloid (S)-reticuline—seven enzymatic steps upstream of codeinone—accumulated in transgenic plants at the expense of morphine, codeine, oripavine and thebaine. Methylated derivatives of reticuline also accumulated. Analysis verified… Expand
Systematic knockdown of morphine pathway enzymes in opium poppy using virus-induced gene silencing.
TLDR
The silencing of alkaloid biosynthetic genes using VIGS confirms the physiological function of enzymes previously characterized in vitro, provides insight into the biochemical regulation of morphine biosynthesis, and demonstrates the immense potential for metabolic engineering in opium poppy. Expand
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TLDR
Discovery of REPI completes the isolation of genes responsible for known steps of morphine biosynthesis, and reduces levels of (R)-reticuline and morphinan alkaloids and increased the overall abundance of (S-reticULine and its O-methylated derivatives. Expand
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TLDR
Two cycles of crossing of the best transgenic line into an elite high-morphine genotype resulted in significant increases in morphine and total alkaloids relative to the elite recurrent parent, and no significant changes in alkaloid profiles or quantities were observed in leaf, roots, pollen and seed. Expand
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Comparative transcriptome analysis of opium poppy and several other Papaver species that do not accumulate morphinan alkaloids showed that known genes encoding BIA biosynthetic enzymes are expressed at higher levels in P. somniferum. Expand
Codeinone reductase isoforms with differential stability, efficiency and product selectivity in opium poppy
TLDR
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The study of transient coover expression of Cor and TYDC genes on morphine accumulation in Papaver somniferum L
Opium poppy (P.somniferum L.) generally contains about 18 tetra hydrobenzylisoquinoline alkaloids . Benzilisoquinoline alkaloids are a diverse group of nitrogen based compounds that are found in 20%Expand
Mutagenesis as a Functional Genomics Platform for Pharmaceutical Alkaloid Biosynthetic Gene Discovery in Opium Poppy
TLDR
An effective functional genomics initiative based on induced mutagenesis and recently developed reverse genetics methodology is established, with the aim of identifying biosynthetic genes that can be used to engineer opium poppy for the production of copious levels of high-value pharmaceutical alkaloids. Expand
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