RNAi-mediated pinoresinol lariciresinol reductase gene silencing in flax (Linum usitatissimum L.) seed coat: consequences on lignans and neolignans accumulation.

@article{Renouard2014RNAimediatedPL,
  title={RNAi-mediated pinoresinol lariciresinol reductase gene silencing in flax (Linum usitatissimum L.) seed coat: consequences on lignans and neolignans accumulation.},
  author={Sullivan Renouard and Marie-Aude Tribalatc and Fr{\'e}d{\'e}ric Lamblin and Ga{\"e}lle Mongelard and Oph{\'e}lie Fliniaux and Cyrielle Corbin and Djurdjica Marosevic and Serge Pilard and Herv{\'e} Demailly and Laurent Gutierrez and Christophe Hano and François Mesnard and {\'E}ric Lain{\'e}},
  journal={Journal of plant physiology},
  year={2014},
  volume={171 15},
  pages={1372-7}
}
RNAi technology was applied to down regulate LuPLR1 gene expression in flax (Linum usitatissimum L.) seeds. This gene encodes a pinoresinol lariciresinol reductase responsible for the synthesis of (+)-secoisolariciresinol diglucoside (SDG), the major lignan accumulated in the seed coat. If flax lignans biological properties and health benefits are well documented their roles in planta remain unclear. This loss of function strategy was developed to better understand the implication of the PLR1… CONTINUE READING
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