RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids.

@article{Lackey2010RNAimediatedDO,
  title={RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids.},
  author={Erika Lackey and Danny W-K Ng and Z. Jeffrey Chen},
  journal={The New phytologist},
  year={2010},
  volume={186 1},
  pages={
          207-15
        }
}
Both natural and newly synthesized allopolyploids display nonadditive gene expression changes through genetic and epigenetic mechanisms. The nonadditively expressed genes include many microRNA (miRNA) targets, suggesting a role for miRNAs and their targets in morphological variation in the allopolyploids and their progenitors. We produced dominant-negative transgenic allotetraploid plants in Arabidopsis using RNA interference (RNAi) that downregulates the expression of miRNA biogenesis genes… CONTINUE READING
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