RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

Abstract

To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants… (More)
DOI: 10.1186/2193-1801-1-74

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