RNAi in Drosophila S2 cells as a tool for studying cell cycle progression.

@article{BettencourtDias2009RNAiID,
  title={RNAi in Drosophila S2 cells as a tool for studying cell cycle progression.},
  author={M{\'o}nica Bettencourt-Dias and Gohta Goshima},
  journal={Methods in molecular biology},
  year={2009},
  volume={545},
  pages={39-62}
}
Genetic studies on model organisms, particularly yeasts and Drosophila melanogaster, have proven powerful in identifying the cell cycle machinery and its regulatory mechanisms. In more recent years RNAi has been used in a variety of genome-wide screens and single molecule studies to elucidate the mechanisms of cell cycle progression. In Drosophila cultured cells, RNAi is extremely simple, and a strong effect can be observed by adding the dsRNA to the cultured cells, with few complications of… CONTINUE READING

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