RNAi Methodologies for the Functional Study of Signaling Molecules

  title={RNAi Methodologies for the Functional Study of Signaling Molecules},
  author={Gwang Lee and Leah A. Santat and Mi Sook Chang and Sangdun Choi},
  journal={PLoS ONE},
  pages={611 - 620}
RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW 264.7 murine macrophage cell line. The specificity of the different RNA interference (RNAi) platforms… CONTINUE READING
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