RNA synthesis during infection by Hendra virus: an examination by quantitative real-time PCR of RNA accumulation, the effect of ribavirin and the attenuation of transcription

@article{Wright2004RNASD,
  title={RNA synthesis during infection by Hendra virus: an examination by quantitative real-time PCR of RNA accumulation, the effect of ribavirin and the attenuation of transcription},
  author={Peter J. Wright and Gary S Crameri and Bryan T. Eaton},
  journal={Archives of Virology},
  year={2004},
  volume={150},
  pages={521-532}
}
Summary.Hendra virus is one of two virus species within the newly-formed genus Henipavirus, subfamily Paramxyovirinae. It is a designated select agent with potential biosecurity threat to both human and animal health. Quantitative real-time PCR was used to measure viral RNA synthesis in Vero cells infected by Hendra virus, and to examine the inhibitory effect of ribavirin. It was also used to determine the points of attenuation during transcription of the six viral genes N, P, M, F, G and L by… Expand
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References

SHOWING 1-10 OF 29 REFERENCES
Viral RNA Mutations Are Region Specific and Increased by Ribavirin in a Full-Length Hepatitis C Virus Replication System
TLDR
A novel hepatitis C virus (HCV) replication system is used to determine relative mutation frequencies in variable and conserved regions of the HCV genome and ribavirin broadly increased error generation, indicating that it acts as an HCV RNA mutagen in vivo and suggesting the replication model may be useful for identifying agents capable of driving replicating virus into error catastrophe. Expand
Hepatitis C Virus RNA-dependent RNA Polymerase (NS5B) as a Mediator of the Antiviral Activity of Ribavirin*
TLDR
Data suggest that ribavirin can exert a direct effect on HCV replication, which is mediated by the HCV RNA polymerase, and the implications of this work on the development of nucleoside analogs for treatment of HCV infection. Expand
Sendai Virus Gene Start Signals Are Not Equivalent in Reinitiation Capacity: Moderation at the Fusion Protein Gene
TLDR
The polar attenuation of SeV transcription appeared to be not linear but biphasic, and the unique F start sequence as well as the other start sequences are perfectly conserved in all SeV isolates sequenced to date, with a virulence several magnitudes lower than that of the fresh isolates. Expand
Order of transcription of genes of vesicular stomatitis virus.
  • L. A. Ball, C. White
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1976
TLDR
The polar effect of UV irradiation is inconsistent with independent transcription of each of the genes of VSV, suggesting that trnascription initiates at a single point on VSV RNA and proceeds in the order 3'-N-NS-M-G-(L)-5'. Expand
Ribavirin Causes Error Catastrophe during Hantaan Virus Replication
TLDR
Results suggest that ribavirin's mechanism of action lies in challenging the fidelity of the hantavirus polymerase, which causes error catastrophe. Expand
Sendai virus gene expression in lytically and persistently infected cells.
TLDR
A cell-associated state of infection seems to be achievable by a relatively weak general reduction of the copy numbers of viral mRNA and genomic RNA. Expand
Molecular characterization of the polymerase gene and genomic termini of Nipah virus.
TLDR
The comparison of the structures of the genomes of HV and NV is now complete and this information will help to establish the taxonomic position of these novel viruses within the family Paramyxoviridae. Expand
RNA virus error catastrophe: Direct molecular test by using ribavirin
TLDR
It is demonstrated that ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material, suggesting that RNA virus mutagens may represent a promising new class of antiviral drugs. Expand
The Exceptionally Large Genome of Hendra Virus: Support for Creation of a New Genus within the FamilyParamyxoviridae
TLDR
The large genome size (18,234 nucleotides), the unique complementary genome terminal sequences of HeV, and the limited homology with other members of the Paramyxoviridae suggest that HeV should be classified in a new genus in this family. Expand
Altered transcription of a defective measles virus genome derived from a diseased human brain.
TLDR
Although the analysed SSPE‐derived defective MV showed a localized transcription defect at the phosphoprotein‐‐matrix gene junction, the corresponding intergenic ‘consensus’ sequence and the surrounding nucleotides were not altered, this implies that factor(s) involved in the transcription of this defective S SPE virus fail to recognize this particular signal sequence, a constellation which in this case might be causally related to the development of MV persistence. Expand
...
1
2
3
...