RNA structure analysis of viruses using SHAPE.

Abstract

Selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) provides a means to investigate RNA structure with better resolution and higher throughput than has been possible with traditional methods. We present several protocols, which are based on a variety of previously published methods and were adapted and optimized for the analysis of… (More)
DOI: 10.1002/9780471729259.mc15h03s30

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