RNA stem-loop enhanced expression of previously non-expressible genes.

@article{Paulus2004RNASE,
  title={RNA stem-loop enhanced expression of previously non-expressible genes.},
  author={Michael J. Paulus and Martin Haslbeck and Manfred Watzele},
  journal={Nucleic acids research},
  year={2004},
  volume={32 9},
  pages={e78}
}
The key step in bacterial translation is formation of the pre-initiation complex. This requires initial contacts between mRNA, fMet-tRNA and the 30S subunit of the ribosome, steps that limit the initiation of translation. Here we report a method for improving translational initiation, which allows expression of several previously non-expressible genes. This method has potential applications in heterologous protein synthesis and high-throughput expression systems. We introduced a synthetic RNA… CONTINUE READING

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