RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii.

@article{Godman2010RNASO,
  title={RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii.},
  author={James E Godman and Attila Moln{\'a}r and David C. Baulcombe and Janneke Balk},
  journal={The Biochemical journal},
  year={2010},
  volume={431 3},
  pages={345-51}
}
The genome of the green alga Chlamydomonas reinhardtii encodes two [FeFe]-hydrogenases, HydA1 and HydA2, and the hydrogenase-like protein HYD3. The unique combination of these proteins in one eukaryotic cell allows for direct comparison of their in vivo functions, which have not been established for HydA2 and HYD3. Using an artificial microRNA silencing method developed recently, the expression of HydA1, HydA2 and HYD3 was specifically down-regulated. Silencing of HydA1 resulted in 4-fold lower… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 50 references

An allelic mutant

D. G. 34 Bernard, Y. Cheng, Y. Zhao, J. Balk
2009

Chlamydomonas reinhardtii: anoxic gene expression, hydrogenase induction, and metabolic pathways

J. Jacobs, S. Pudollek, A. Hemschemeier, T. Happe
J. Biol. Chem. 282, • 2009

Crucial role of conserved cysteine residues in the assembly of two iron-sulfur clusters on the CIA protein

E. Urzica, A. Pierik, U. Mühlenhoff, R. Lill
Nar1. Biochemistry • 2009

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