RNA sequencing of FACS-sorted immune cell populations from zebrafish infection models to identify cell specific responses to intracellular pathogens.

@article{Rougeot2014RNASO,
  title={RNA sequencing of FACS-sorted immune cell populations from zebrafish infection models to identify cell specific responses to intracellular pathogens.},
  author={Julien Rougeot and Ania Zakrzewska and Zakia Kanwal and Hans J. Jansen and Herman P. Spaink and Annemarie H. Meijer},
  journal={Methods in molecular biology},
  year={2014},
  volume={1197},
  pages={
          261-74
        }
}
The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live imaging of interactions between pathogens and host cells. A number of useful fluorescent reporter lines have recently been developed and significant advances in RNA sequencing technology have been made, which now make it possible to apply the zebrafish model for investigating changes in… CONTINUE READING
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