RNA sequence variants in live poliovirus vaccine and their relation to neurovirulence.

@article{Chumakov1992RNASV,
  title={RNA sequence variants in live poliovirus vaccine and their relation to neurovirulence.},
  author={Konstantin Chumakov and Laurie P Norwood and Mary L. Parker and Eugenia M. Dragunsky and Yongkang Ran and Inessa S. Levenbook},
  journal={Journal of virology},
  year={1992},
  volume={66 2},
  pages={
          966-70
        }
}
Mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC) was used to study sequence heterogeneity and stability in attenuated poliovirus type 3 at positions in which the vaccine virus differs from its wild-type progenitor. Of seven genomic positions tested, only two (positions 472 and 2493) show nucleotide heterogeneity. Propagation of the vaccine virus in cell cultures leads to rapid selection of virus with reversions at these two positions of the genome. The… CONTINUE READING

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