RNA processing and ribonucleoprotein assembly studied in vivo by RNA transfection.

@article{Kleinschmidt1990RNAPA,
  title={RNA processing and ribonucleoprotein assembly studied in vivo by RNA transfection.},
  author={Ann Kleinschmidt and Thoru Pederson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 4},
  pages={1283-7}
}
We present a method for studying RNA processing and ribonucleoprotein assembly in vivo, by using RNA synthesized in vitro. SP6-transcribed 32P-labeled U2 small nuclear RNA precursor molecules were introduced into cultured human 293 cells by calcium phosphate-mediated uptake, as in standard DNA transfection experiments. RNase protection mapping demonstrated that the introduced pre-U2 RNA underwent accurate 3' end processing. The introduced U2 RNA was assembled into ribonucleoprotein particles… CONTINUE READING

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Nucleic Acids Res . 17 , 48174828

  • E. A. Lerner, M. R. Lerner, C. A. Janeway, J. A. Steitz
  • Proc . Natl . Acad . Sci . USA
  • 1989

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