RNA polymerase II ternary complexes may become arrested after transcribing to within 10 bases of the end of linear templates.

@article{Izban1995RNAPI,
  title={RNA polymerase II ternary complexes may become arrested after transcribing to within 10 bases of the end of linear templates.},
  author={M G Izban and I Samkurashvili and Donal S Luse},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 5},
  pages={2290-7}
}
In the presence of elongation factor SII, arrested RNA polymerase II ternary complexes cleave 7-17 nucleotides from the 3'-ends of their nascent RNAs. It has been shown that transcription of linear templates generates apparent run-off RNAs, which are nevertheless truncated upon incubation with SII. By using high resolution gels, we demonstrate that transcription of blunt or 3'-overhung templates with RNA polymerase II generates two populations of ternary complexes. The first class pauses 5-10… CONTINUE READING

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