RNA polymerase I catalysed transcription of insert viral cDNA.

@article{Zobel1993RNAPI,
  title={RNA polymerase I catalysed transcription of insert viral cDNA.},
  author={A. Zobel and George Bradford Neumann and Gerd Hobom},
  journal={Nucleic acids research},
  year={1993},
  volume={21 16},
  pages={
          3607-14
        }
}
RNA polymerase I has been used for transcription of influenza hemagglutinin (HA) cDNA precisely linked in the anti-sense configuration to both mouse rDNA promoter and terminator segments. In transcription reactions based on Ehrlich ascites cell nuclear extracts, specific uniform RNA products are synthesized in high rates that are comparable to original rDNA template transcriptions. Primer extension reactions show the 5' ends of these RNA transcripts to be located exactly at position +1… CONTINUE READING

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