RNA minihelices as model substrates for ATP/CTP:tRNA nucleotidyltransferase.

  title={RNA minihelices as model substrates for ATP/CTP:tRNA nucleotidyltransferase.},
  author={Zao-ying Li and Yin Guang Sun and David L. Thurlow},
  journal={The Biochemical journal},
  volume={327 ( Pt 3)},
Twenty-one RNA minihelices, resembling the coaxially stacked acceptor- /T-stems and T-loop found along the top of a tRNA's three-dimensional structure, were synthesized and used as substrates for ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and Saccharomyces cerevisiae. The sequence of nucleotides in the loop varied at positions corresponding to residues 56, 57 and 58 in the T-loop of a tRNA. All minihelices were substrates for both enzymes, and the identity of bases in the loop… CONTINUE READING


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