RNA isolation from soil for bacterial community and functional analysis: evaluation of different extraction and soil conservation protocols.

@article{Sessitsch2002RNAIF,
  title={RNA isolation from soil for bacterial community and functional analysis: evaluation of different extraction and soil conservation protocols.},
  author={Angela Sessitsch and Stephen Gyamfi and Nancy Stralis-Pavese and Alexandra Weilharter and Ulrike Pfeifer},
  journal={Journal of microbiological methods},
  year={2002},
  volume={51 2},
  pages={171-9}
}
The impact of three different RNA isolation methods on the community analysis of metabolically active bacteria was determined by reverse transcription (RT) and PCR amplification of 16S rRNA genes and subsequent terminal restriction fragment length polymorphism (T-RFLP) analysis. Furthermore, soil samples were stored at different conditions in order to evaluate the effect of soil conservation methods on the outcome of the population analysis. The quality of mRNA was assessed by reverse… CONTINUE READING

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