RNA isolation from embryonic zebrafish and cDNA synthesis for gene expression analysis.

@article{Peterson2009RNAIF,
  title={RNA isolation from embryonic zebrafish and cDNA synthesis for gene expression analysis.},
  author={Samuel Mark Peterson and Jennifer L Freeman},
  journal={Journal of visualized experiments : JoVE},
  year={2009},
  volume={30}
}
Many important and complex laboratory procedures require an input of high quality, intact RNA. A degraded sample or the presence of impurities can lead to disastrous results in downstream experimental applications. It is therefore, of utmost importance to use solid techniques with numerous safeguards and quality control checks to ensure a superior sample. Herein, we detail a protocol to isolate total RNA from whole zebrafish embryos using a commercially available chemical denaturant and… CONTINUE READING

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