RNA interference reveals a requirement for myocyte enhancer factor 2A in activity-dependent neuronal survival.

@article{Gaudillire2002RNAIR,
  title={RNA interference reveals a requirement for myocyte enhancer factor 2A in activity-dependent neuronal survival.},
  author={Brice Gaudilli{\`e}re and Yang Shi and Azad Bonni},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 48},
  pages={46442-6}
}
RNA interference (RNAi) provides a powerful method of gene silencing in eukaryotic cells, including proliferating mammalian cells. However, the utility of RNAi as a method of gene knock-down in primary postmitotic mammalian neurons remained unknown. Here, we asked if RNAi might be utilized to allow the assessment of the biological function of a specific gene in the nervous system. We employed a U6 promoter-driven DNA template approach to induce hairpin RNA-triggered RNAi to characterize the… CONTINUE READING