RNA interference of Xenopus NMDAR NR1 in vitro and in vivo

@article{Miskevich2006RNAIO,
  title={RNA interference of Xenopus NMDAR NR1 in vitro and in vivo},
  author={Frank Miskevich and John G. Doench and Matthew Townsend and Phillip A. Sharp and Martha Constantine‐Paton},
  journal={Journal of Neuroscience Methods},
  year={2006},
  volume={152},
  pages={65-73}
}
Conserved Expression of the Glutamate NMDA Receptor 1 Subunit Splice Variants during the Development of the Siberian Hamster Suprachiasmatic Nucleus
TLDR
Examination of the expression of NR1 isoforms in the hamster at different developmental ages suggests that a switch inNR1 isoform does not underlie or is not produced by developmental changes within the hamsters SCN, an important aspect of the function of the SCN as a responder to environmental changes in quality of light over the circadian day and annual cycle.
Application of the RNA interference technique to Xenopus embryos: Specific reduction of the β‐catenin gene products by short double‐stranded RNA produced by recombinant human Dicer
TLDR
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Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus
TLDR
Electroporation can be used as a versatile tool to investigate molecular pathways involved in axon extension during Xenopus embryogenesis and can be tailored easily to other developing organ systems and to other organisms by making simple adjustments to the electroporation chamber.
Xenopus NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1
TLDR
This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1-mediated gliogenesis in Xenopus retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenic.
Xenopus NM 23-X 4 regulates retinal gliogenesis through interaction with p 27 Xic 1
TLDR
This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1mediated gliogenesis in Xenopus retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenic.
New technology for an old favorite: lentiviral transgenesis and RNAi in rats
TLDR
The powerful combination of lentiviral vectors with RNA interference (RNAi), which allows one to produce transgenic animals expressing short hairpin RNA (shRNA) to “knock down” specific gene expression, is reviewed, highlighting the power of in vivo RNAi for creating new genetic models in the rat.
Experience-dependent regulation of presynaptic NMDA receptors (preNMDARs) and their role in neurotransmission and synaptic plasticity
TLDR
This dissertation will attempt to explain how preNMDARs are tonically active and how this can explain their developmental and possibly experience-dependent modifications.
Induction of Vertebrate Regeneration by a Transient Sodium Current
TLDR
A previously undetected competency window in which cells retain their intrinsic regenerative program is revealed, a novel endogenous role for NaV in regeneration is identified, and modulation of sodium transport represents an exciting new approach to organ repair.
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