RNA interference of Xenopus NMDAR NR1 in vitro and in vivo

  title={RNA interference of Xenopus NMDAR NR1 in vitro and in vivo},
  author={Frank Miskevich and John G. Doench and Matthew Townsend and Phillip A. Sharp and Martha Constantine‐Paton},
  journal={Journal of Neuroscience Methods},

Co-expression of Argonaute2 Enhances Short Hairpin RNA-induced RNA Interference in Xenopus CNS Neurons In Vivo

It is suggested that co-expression of Ago2 and shRNA is a simple method to enhance RNAi in intact animals, and this should extend the application of RNAi to study gene function of intact brain circuits.

Conserved Expression of the Glutamate NMDA Receptor 1 Subunit Splice Variants during the Development of the Siberian Hamster Suprachiasmatic Nucleus

Examination of the expression of NR1 isoforms in the hamster at different developmental ages suggests that a switch inNR1 isoform does not underlie or is not produced by developmental changes within the hamsters SCN, an important aspect of the function of the SCN as a responder to environmental changes in quality of light over the circadian day and annual cycle.

Application of the RNA interference technique to Xenopus embryos: Specific reduction of the β‐catenin gene products by short double‐stranded RNA produced by recombinant human Dicer

The results strongly suggest that the RNAi technique can be applied to Xenopus embryos using short dsRNAs, appropriate temperature control, and proper selection of target genes.

Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus

Electroporation can be used as a versatile tool to investigate molecular pathways involved in axon extension during Xenopus embryogenesis and can be tailored easily to other developing organ systems and to other organisms by making simple adjustments to the electroporation chamber.

Xenopus NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1

This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1-mediated gliogenesis in Xenopus retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenic.

Xenopus NM 23-X 4 regulates retinal gliogenesis through interaction with p 27 Xic 1

This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1mediated gliogenesis in Xenopus retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenic.

Experience-dependent regulation of presynaptic NMDA receptors (preNMDARs) and their role in neurotransmission and synaptic plasticity

This dissertation will attempt to explain how preNMDARs are tonically active and how this can explain their developmental and possibly experience-dependent modifications.

Induction of Vertebrate Regeneration by a Transient Sodium Current

A previously undetected competency window in which cells retain their intrinsic regenerative program is revealed, a novel endogenous role for NaV in regeneration is identified, and modulation of sodium transport represents an exciting new approach to organ repair.



Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo

A method for genetic manipulation and subsequent phenotypic analysis of individual cortical neurons in vivo is established and is ideally suited for analysis of gene functions in individual neurons in the intact brain.

Long-term survival, migration, and differentiation of neural cells without functional NMDA receptors in vivo.

Evidence is provided that NMDA-receptor-initiated signals are not required for the postnatal differentiation and survival of many types of neurons in the central nervous system, in a noncell autonomous fashion after transplantation into a wild-type environment.

Studies of NMDA Receptor Function and Stoichiometry with Truncated and Tandem Subunits

It is shown here that truncated NMDA receptor subunits may be partially rescued by coexpressing the final TM and tail as a separate protein, and may redirect future studies into the mechanism of binding and gating in these receptors toward schemes including dimers.

Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons

It is concluded that ionotropic glutamate receptors are regulated in native neurons by rapid, subtype-specific membrane trafficking, which may modulate synaptic transmission in response to physiological or pathophysiological activation.

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.

Chronic NMDA receptor blockade from birth delays the maturation of NMDA currents, but does not affect AMPA/kainate currents.

The present data show that NR function is necessary for subsequent NR current regulation in vivo, but it is not essential for the developmental expression of normal AMPAR currents.

Interaction between Metabotropic and NMDA Subtypes of Glutamate Receptors in Sprout Suppression at Young Synapses

It is shown that type II metabotropic glutamate receptors (mGluRs) can operate synergistically with NMDA receptors in the absence of AMPA/KA receptor function to suppress an early neurite sprouting response of the tectal neurons.

A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

It is shown that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes.