RNA interference of Xenopus NMDAR NR1 in vitro and in vivo

@article{Miskevich2006RNAIO,
  title={RNA interference of Xenopus NMDAR NR1 in vitro and in vivo},
  author={Frank Miskevich and John G. Doench and Matthew Townsend and Phillip A. Sharp and Martha Constantine-Paton},
  journal={Journal of Neuroscience Methods},
  year={2006},
  volume={152},
  pages={65-73}
}
Here, we present a short interfering RNA (siRNA) application that reduces the expression of NR1 the obligate subunit of the NMDA receptor (NMDAR) and virtually eliminates NMDAR function in a small subset of neurons within otherwise normally developing Xenopus laevis tadpoles. We designed two plasmids each containing a CMV promoter driving a dsRed "reporter" cDNA and DNA coding for one short hairpin RNA (shRNA) under the control of the U6 promoter. The shRNA was cleaved to produce a siRNA… Expand
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