RNA interference of PPARγ using fiber-modified adenovirus vector efficiently suppresses preadipocyte-to-adipocyte differentiation in 3T3-L1 cells

@article{Hosono2005RNAIO,
  title={RNA interference of PPAR$\gamma$ using fiber-modified adenovirus vector efficiently suppresses preadipocyte-to-adipocyte differentiation in 3T3-L1 cells},
  author={Tetsuji Hosono and Hiroyuki Mizuguchi and Kazufumi Katayama and Naoya Koizumi and Kenji Kawabata and Teruhide Yamaguchi and Shinsaku Nakagawa and Yoshiteru Watanabe and Tadanori Mayumi and Takao Hayakawa},
  journal={Gene},
  year={2005},
  volume={348},
  pages={157-165}
}
Abstract The peroxisome proliferator-activated receptor (PPAR) γ is regarded as a “master regulator” of adipocyte differentiation and is abundantly expressed in adipose. To understand the biological role of PPARγ in adipose, RNA interference (RNAi) of PPARγ should be a powerful tool. 3T3-L1 cell line serves an excellent model to investigate the mechanism of preadipocyte-to-adipocyte differentiation. However, this cell line is difficult to transfect by plasmid vectors and viral vectors. We… Expand
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