RNA interference is an antiviral defence mechanism in Caenorhabditis elegans

@article{Wilkins2005RNAII,
  title={RNA interference is an antiviral defence mechanism in Caenorhabditis elegans},
  author={Courtney Wilkins and Ryan Dishongh and Steve C. Moore and Michael A. Whitt and Marie Chow and Khaled Machaca},
  journal={Nature},
  year={2005},
  volume={436},
  pages={1044-1047}
}
RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-transcriptional gene silencing mechanism that is well defined genetically in Caenorhabditis elegans. RNAi has been postulated to function as an adaptive antiviral immune mechanism in the worm, but there is no experimental evidence for this. Part of the limitation is that there are no known natural viral pathogens of C. elegans. Here we describe an infection model in C. elegans using the mammalian pathogen vesicular… Expand
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References

SHOWING 1-10 OF 30 REFERENCES
Inhibition of Retroviral Pathogenesis by RNA Interference
TLDR
Analysis of the step of the retroviral life cycle that is inhibited by RNAi revealed that it primarily prevented accumulation of the viral RNAs synthesized late during infection. Expand
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
TLDR
It is shown that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates and the possibility that one natural function of RNAi is transposon silencing is discussed. Expand
Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing.
  • W. Li, Hongwei Li, +9 authors S. Ding
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
TLDR
These findings provide evidence that mammalian virus proteins can inhibit RNA silencing, implicating this mechanism as a nucleic acid-based antiviral immunity in mammalian cells. Expand
Short interfering RNA confers intracellular antiviral immunity in human cells
TLDR
It is demonstrated that dsRNA can effectively protect human cells against infection by a rapidly replicating and highly cytolytic RNA virus and elicit specific intracellular antiviral resistance that may provide a therapeutic strategy against human viruses. Expand
Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi
TLDR
It is shown that loss of function of a putative RNA-directed RNA polymerase (RdRP) of C. elegans, RRF-3, results in a substantial enhancement of sensitivity to RNAi in diverse tissues. Expand
Evidence that HIV-1 encodes an siRNA and a suppressor of RNA silencing.
TLDR
The evidence that HIV-1 encodes viral siRNA precursors in its genome is reported and that natural HIV- 1 infection provokes nucleic acid-based immunity in human cells is reported. Expand
RNAi (Nematodes: Caenorhabditis elegans).
TLDR
This chapter describes the history of the discovery of RNAi, its systemic nature, inheritance, and connection to other homology-dependent silencing phenomena like co-suppression and transcriptional gene silencing, and discusses RNAi-deficient mutants in C. elegans. Expand
Genes Required for Systemic RNA Interference in Caenorhabditis elegans
TLDR
This work reports the characterization of mutants defective in the systemic aspect of RNAi, but not in the core RNAi process itself, and suggests that dsRNA uptake is a specific process involving several unique proteins. Expand
A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
TLDR
It is shown that C. elegans eri-1 encodes an evolutionarily conserved protein with domains homologous to nucleic-acid-binding and exonuclease proteins that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference. Expand
RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome
TLDR
It is demonstrated that dsRNA-mediated interference also can act as a viral defense mechanism in mosquito cells, consistent with RNA silencing as the mechanism of resistance to DEN-2 in transformed mosquito cells. Expand
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