RNA interference in parasitic helminths: current situation, potential pitfalls and future prospects

@article{Geldhof2006RNAII,
  title={RNA interference in parasitic helminths: current situation, potential pitfalls and future prospects},
  author={Peter Eduard Geldhof and Aline Visser and D Clark and Gary I. Saunders and Collette Britton and John. S. Gilleard and Matt Berriman and David P. Knox},
  journal={Parasitology},
  year={2006},
  volume={134},
  pages={609 - 619}
}
SUMMARY RNA interference (RNAi) has become an invaluable tool for the functional analysis of genes in a wide variety of organisms including the free-living nematode Caenorhabditis elegans. Recently, attempts have been made to apply this technology to parasitic helminths of animals and plants with variable success. Gene knockdown has been reported for Schistosoma mansoni by soaking or electroporating different life-stages in dsRNA. Similar approaches have been tested on parasitic nematodes which… Expand
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References

SHOWING 1-10 OF 50 REFERENCES
Testing the efficacy of RNA interference in Haemonchus contortus.
TLDR
Findings suggest that the RNAi pathway is functional in H. contortus and that, under certain conditions, it is possible to suppress gene expression by RNAi, but it only works on a limited number of genes and the effect is small and difficult to reproduce. Expand
Efficacy and specificity of RNA interference in larval life-stages of Ostertagia ostertagi
TLDR
The results indicate that the RNAi pathway is probably present in O. ostertagi but that the current RNAi techniques can not be used as a reliable screening method. Expand
RNA interference in protozoan parasites
RNA interference or RNAi is defined as the mechanism through which gene‐specific, double‐stranded RNA (dsRNA) triggers degradation of homologous transcripts. Besides providing an invaluable tool toExpand
Development of methods for RNA interference in the sheep gastrointestinal parasite, Trichostrongylus colubriformis.
TLDR
Feeding was, however, successful with tropomyosin as a target, suggesting that mode of delivery is an important parameter of RNAi, and electroporation is a particularly efficient means of inducing RNA in nematodes with either short dsRNA oligonucleotides or with long in vitro transcribed ds RNA molecules. Expand
Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference.
TLDR
Treatment with dsRNA corresponding to the major sperm protein (MSP) had no effect on either parasite development or sexual fate over 14 days, establishing a procedure for RNAi of plant parasitic nematodes. Expand
RNA interference of dual oxidase in the plant nematode Meloidogyne incognita.
TLDR
In planta infection data show that a single 4-h preinfection treatment with double-stranded RNA derived from the peroxidase region of a dual oxidase gene has effects on gene expression that are phenotypically observable 35 days postinfection. Expand
RNA interference and plant parasitic nematodes.
TLDR
RNAi should help identify gene and, hence, protein targets for nematode control strategies and prospects for novel resistance depend on the plant generating an effective form of double-stranded RNA in the absence of an endogenous target gene without detriment to itself. Expand
Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts.
TLDR
RNA-interference will enable direct assessment of the roles of individual genes in physiological processes of larval stages of S. mansoni, a crucial step in the identification of novel intervention targets for this important human pathogen. Expand
Suppression of secreted acetylcholinesterase expression in Nippostrongylus brasiliensis by RNA interference.
TLDR
Whether RNAi could be applied to the parasitic nematode Nippostrongylus brasiliensis, utilising secreted acetylcholinesterases (AChEs) as targets was tested, and it was found that the RNAi effect can be remarkably long-lived. Expand
Transgene expression in Schistosoma mansoni: introduction of RNA into schistosomula by electroporation.
TLDR
The finding that electroporation can be used to introduce RNA into schistosomula raises the possibility of using this approach to introduce either DNA constructs or dsRNA sequences, both of which might be expected to have longer-term, ideally inheritable, effects. Expand
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