RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo

@article{Nishiyama2008RNAIB,
  title={RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo},
  author={A Nishiyama and Shigeki Fujiwara},
  journal={Development},
  year={2008},
  volume={50}
}
We carried out RNA interference by expressing short hairpin RNA (shRNA) in the Ciona intestinalis embryo. For this purpose, we identified a gene encoding U6 small nuclear RNA (snRNA) in the C. intestinalis genome. The 1‐kb sequence upstream of the U6 snRNA gene was sufficient for directing transcription of short RNA as revealed by Northern blot hybridization. An shRNA‐expressing plasmid vector was constructed, in which shRNA‐encoding oligonucleotides are inserted downstream of the U6 promoter… 
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