RNA interference by 2',5'-linked nucleic acid duplexes in mammalian cells.

@article{Prakash2006RNAIB,
  title={RNA interference by 2',5'-linked nucleic acid duplexes in mammalian cells.},
  author={Thazha P. Prakash and Bryan A Kraynack and Brenda F. Baker and Eric E Swayze and B. Shyamasundara Bhat},
  journal={Bioorganic & medicinal chemistry letters},
  year={2006},
  volume={16 12},
  pages={3238-40}
}
Synthetic small interfering RNA (siRNA) mediated silencing of a specific gene is emerging as a powerful tool for gene regulation. However, their utility is limited for therapeutic applications primarily due to poor stability. The 2',5'-linked oligonucleotides are known to be more stable to nucleolytic degradation than 3',5'-linked oligonucleotides. The 2',5'-linkage is tolerated in the sense strand of the siRNA duplex. However, the 2',5'-linkage is not tolerated in the antisense strand of the… CONTINUE READING