RNA interference: potential therapeutic targets
@article{Jana2004RNAIP, title={RNA interference: potential therapeutic targets}, author={Snehasis Jana and Chaitaly T. Chakraborty and Shivani Nandi and J. K. Deb}, journal={Applied Microbiology and Biotechnology}, year={2004}, volume={65}, pages={649-657} }
One of the most exciting findings in recent years has been the discovery of RNA interference (RNAi). RNAi methodologies hold the promise to selectively inhibit gene expression in mammals. RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences, including endogenous mRNAs. The use of…
46 Citations
Illuminating the Gateway of Gene Silencing: Perspective of RNA Interference Technology in Clinical Therapeutics
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The recent discoveries associated with the phenomenon of RNAi, expression of silencing molecules in mammals along with the vector systems used for disease therapeutics are highlighted.
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Nonviral methods for siRNA delivery.
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The sense strand pre-cleaved RNA duplex mediates an efficient RNA interference with less off-target and immune response effects
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- 2010
It is reported that RNA duplex with the sense strand pre-cleaved at the base between base 10 and 11 relative to the 5′ end of the antisense strand induced a target-specific RNA silencing effectively.
BARRIERS TO SUCCESSFUL DELIVERY OF SHORT INTERFERING RNA AFTER SYSTEMIC ADMINISTRATION
- BiologyClinical and experimental pharmacology & physiology
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The present review considers the biological barriers that limit the movement of siRNA from vascular lumen to target cell cytoplasm and the strategies that have been used to overcome them.
Off-target possibility of efficient siRNA using String matching
- Biology2017 International Conference on Circuit ,Power and Computing Technologies (ICCPCT)
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This work uses string matching algorithm in GPU instead of BLAST to design an efficient exogenous siRNA and the original experimental value of Huesken data set is used to evaluate the current work.
Cationic lipids and polymers mediated vectors for delivery of siRNA.
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