RNA interference: a promising technique for the improvement of traditional crops

  title={RNA interference: a promising technique for the improvement of traditional crops},
  author={Rajan Katoch and Neelam Thakur},
  journal={International Journal of Food Sciences and Nutrition},
  pages={248 - 259}
  • R. Katoch, N. Thakur
  • Published 1 February 2013
  • Biology, Medicine
  • International Journal of Food Sciences and Nutrition
RNA interference (RNAi) is a homology-dependent gene-silencing technology that involves double-stranded RNA directed against a target gene. This technique has emerged as powerful tool in understanding the functions of a number of genes in recent years. For the improvement in the nutritional status of the plants and reduction in the level of antinutrients, the conventional breeding methods were not completely successful in achieving the tissue-specific regulation of some genes. RNAi has shown… Expand
RNA Interference: A Promising Approach for Crop Improvement
Several desirable traits have been improved in the crop varieties through RNAi, which include crop protection against biotic and abiotic stresses, enhancement of nutritional value, alteration in plant architecture for better adaptation to environmental conditions, overexpression or removal of secondary metabolites, enhancements of shelf life of fruits and vegetables, generation of male sterile lines, and development of seedless fruits. Expand
Knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction.
  • Isabela Tristan Lourenço-Tessutti, J. D. A. D. S. Júnior, +8 authors Maria Fatima Grossi-de-Sa
  • Biology, Medicine
  • Phytopathology
  • 2015
Crop losses caused by nematode infections are estimated to be valued at USD 157 billion per year. Meloidogyne incognita, a root-knot nematode (RKN), is considered to be one of the most importantExpand
Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis
The data presented here demonstrates that A. grandis midgut nucleases are effectively one of the main barriers to dsRNA delivery and emphasize the need to develop novel RNAi delivery strategies focusing on protecting the ds RNA from gut nucleases and enhancing its oral delivery and uptake to crop insect pests. Expand
Cloning, characterization, expression analysis and inhibition studies of a novel gene encoding Bowman-Birk type protease inhibitor from rice bean.
The novel RbTI gene will further broaden the pool of plant defense genes and could be an ideal choice for developing transgenic crops resistant to insect pests with high economic value and has the potential to be used as a probe for selection of insect- and pathogen-resistant genotypes. Expand
Evaluation and characterization of trypsin inhibitor from rice bean with inhibitory activity against gut proteases of Spodoptera litura
The role of rice bean protease inhibitors as a potential strategy against insectpests of economic importance is clarified and the highest TI content in the seeds nearing maturity correlates to the role of TIs in protecting against insect pests. Expand
The Role of Interconversion of Scopoletin and Scopolin in Cassava Postharvest Physiological Deterioration (PPD)
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Role of RNA interference in plant improvement
Manipulating new RNAi pathways, which generate small RNA molecules to amend gene expression in crops, can produce new quality traits and having better potentiality of protection against abiotic and biotic stresses. Expand
RNAi induced gene silencing in crop improvement
  • S. K. Sinha
  • Biology, Medicine
  • Physiology and Molecular Biology of Plants
  • 2010
This review article illustrates the application of RNAi in a broad area of crop improvement where this technology has been successfully used and provides historical perspective ofRNAi discovery and its contemporary phenomena, mechanism of RNA i pathway. Expand
RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering
Recent applications of RNAi and microRNA for improving the nutritional value of plants are reviewed, applications of metabolomics technologies in genetic engineering are discussed, and an update is provided on the related RNA i and micro RNA technologies. Expand
The effects of spacer sequences on silencing efficiency of plant RNAi vectors
The results show the possibility of producing a broad range of the RNAi-induced silencing phenotypes by replacing the spacer sequence of RNAi construct. Expand
Simple RNAi vectors for stable and transient suppression of gene function in rice.
A Gateway vector, pANDA, is developed for RNA interference of rice genes to help identify the functions of genes whose tagged mutants are not available at present and complement existing methods for functional genomics of rice. Expand
Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato
When this fast way of shutting down the ACC oxidase gene was applied, transgenic tomato plants were produced that had fruit which released traces of ethylene and had a prolonged shelf life of more than 120 days. Expand
A high-throughput inducible RNAi vector for plants.
A vector system that allows dexamethasone-inducible RNAi against plant genes and the inducibility of RNAi from this system may be useful in helping to identify the functions of genes which when constitutively silenced give embryo lethality or pleiotropic phenotypes. Expand
Silencing of GmFAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]
It is shown that the transgene-produced siRNA caused silencing of FAD3 that was comparable to the fad3 mutant phenotype and, furthermore, that such a silencing is stably inherited in engineered soybean lines. Expand
[Reducing the maize amylopectin content through RNA interference manipulation].
To regulate the biosynthesis of maize starch, gene segment of starch branch enzyme (SBE) in maize was cloned and an expression vector of small interference RNA with the fragment of antisense gene +Expand
RNA interference in protozoan parasites
RNA interference or RNAi is defined as the mechanism through which gene‐specific, double‐stranded RNA (dsRNA) triggers degradation of homologous transcripts. Besides providing an invaluable tool toExpand