RNA interference: The molecular immune system

  title={RNA interference: The molecular immune system},
  author={Omar Bagasra and Kiley R. Prilliman},
  journal={Journal of Molecular Histology},
Introduction of double-stranded RNA (dsRNA) into cells expressing a homologous gene triggers RNA interference (RNAi), or RNA-based gene silencing (RBGS). The dsRNA degrades corresponding host mRNA into small interfering RNAs (siRNAs) by a protein complex containing Dicer. siRNAs in turn are incorporated into the RNA-induced silencing complex (RISC) that includes helicase, RecA, and exo- and endo-nucleases as well as other proteins. Following its assembly, the RISC guides the RNA degradation… 

Dissection of Double-Stranded RNA Binding Protein B2 from Betanodavirus

It is found that B2 is able to induce apoptosis in fish cells but that this was not the result of dsRNA binding, and that siRNA-mediated knockdown of Dicer dramatically enhanced the accumulation of a B2 mutant.

RNA interference and ion channel physiology

RNAi has provided definitive identification of ion channel subtypes responsible for both basal and stimulated ion conduction across the plasma membrane of several cell types and has been particularly effective in identifying and establishing the contribution of auxiliary subunits and regulatory proteins to the overall function of ionChannel complexes.

The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection

The lack of vsRNA fragments led to the conclusion that during virus infection the siRNA pathway is suppressed by either the cell or the virus itself, although SINV has been shown not to have any RNAi suppressors in previous studies conducted on its insect vector.

RNA silencing movement in plants

This review attempts to clarify commonalities and differences between the individual silencing pathways of RNA silencing spread in plants, and reveals various and sometimes surprising genetic elements of the short‐range silences spread pathway.

Role of Micro-RNAs in Regulation of Lentiviral Latency and Persistence

The data suggest that triplex formation may be an important mechanism of LV latency mediated by endogenous miRNAs, and perinuclear triplexes are formed in LV-infected cells.

Host gene targets for novel influenza therapies elucidated by high‐throughput RNA interference screens

Meta‐analysis of findings from genome‐wide RNAi screens has shown influenza virus to be dependent on functional nodes in host cell pathways, requiring a wide variety of molecules and cellular proteins for replication.

Models for the optical dispersion relations of amorphous semiconductors

A number of heuristic optimization methods for the minimal siRNA covering problem are explored, which include evolutionary heuristics, as well as novel greedy methods applied for the first time to the minimalSiRNA cover problem.

Knockdown of Actin and Caspase Gene Expression by RNA Interference in the Symbiotic Anemone Aiptasia pallida

Since the discovery of the ancient eukaryotic process of RNA-mediated gene silencing, the reverse-genetics technique RNA interference (RNAi) has increasingly been used to examine gene function in

Molecular cloning and immune responsive expression of a ribonuclease III orthologue involved in RNA interference, dicer, in grass carp Ctenopharyngodon idella.

Results collectively implied that the identified cidicer was an inducible gene responding to viral infection both in vitro and in vivo, and the data would shed light on the interaction between RNA interference (RNAi) antiviral pathway and aquareovirus infection.



Gene silencing in mammalian cells by preformed small RNA duplexes.

Modulation of HIV-1 replication by RNA interference

The utility of RNAi for modulating the HIV replication cycle is demonstrated and evidence that genomic HIV-1 RNA, as it exists within a nucleoprotein reverse-transcription complex, is amenable to siRNA-mediated degradation is provided.

Effective expression of small interfering RNA in human cells

The simplicity of the U6 expression cassette and its widespread transcription in human cell types suggest that this mode of siRNA delivery could be useful for suppressing expression of a wide range of genes.

RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase.

This work presents an alternative method to obtain cheap and large amounts of siRNAs using T7 RNA polymerase, and demonstrates silencing of both exogenous and endogenous genes.

RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

  • J. YuS. DeRuiterD. Turner
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 2002
It is found that U6-driven hairpin siRNAs dramatically reduced the expression of a neuron-specific β-tubulin protein during the neuronal differentiation of mouse P19 cells, demonstrating that this approach should be useful for studies of differentiation and neurogenesis.

U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells

A vector-based siRNA expression system that can induce RNAi in mammalian cells is reported, which might allow therapeutic applications by means of vector-mediated RNAi and facilitate a wide range of functional analysis of mammalian genes.

Inhibition of Retroviral Pathogenesis by RNA Interference

RNAi and double-strand RNA.

dsRNA mediated suppression of specific gene expression has also been observed in plants and one demonstration of the phenomenon follows expression in plant cells of a recombinant RNA virus containing exonic sequences of an endogenous cellular gene.

Targeted mRNA degradation by double-stranded RNA in vitro.

The development of a cell-free system from syncytial blastoderm Drosophila embryos that recapitulates many of the features of RNAi is reported, demonstrating that RNAi can be mediated by sequence-specific processes in soluble reactions.

Nucleic Acid-Based Immune System: the Antiviral Potential of Mammalian RNA Silencing

The discovery of a highly conserved cellular machinery that can regulate gene expression in response to double-stranded RNA may revolutionize mammalian virology. This revolution promises not only a