RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.

  title={RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.},
  author={Robert T. Elder and Elwyn Y. Loh and R W Davis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={80 9},
  • R. Elder, E. Loh, R. W. Davis
  • Published 1 May 1983
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
The RNA homologous to the yeast transposable element Ty1 is one of the more abundant poly(A)+ RNAs in many strains of the yeast Saccharomyces cerevisiae. The 5' and 3' ends of Ty1 RNA have been determined from analysis of cDNA. The 5' end is 245 bases into the left delta sequence measured from the left side of the Ty1 element. The delta sequence is a direct repeat of about 340 base pairs present at each end of the Ty1 element. The Ty1 transcription includes 93-97 bases of the left delta… 
The Genetic Organization of the Yeast Ty Element
The genetic organization of the yeast transposon Ty resembles that of higher eukaryotic retroviruses and other elements such as the copia-like sequences of Drosophila and is likely that the Ty-VLPs are units of transposition as Ty transposes via an RNA intermediate.
A region internal to the coding sequences is essential for transcription of the yeast Ty-D15 element
The location and properties of transcription signals for Ty-D15 differ considerably from those expected for a yeast gene transcribed by RNA polymerase II.
Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses
The inferred order of functional domains within TYB3--protease, reverse transcriptase, and endonuclease--resembles the order in Drosophila element 17.6 and in animal retroviruses but is different from that found in yeast elements Ty1 and Ty2.
Complete nucleotide sequence of the Drosophila transposable element copia: homology between copia and retroviral proteins
Comparison of the copia sequence with those of the Saccharomyces cerevisiae transposable element Ty, several vertebrate retroviruses, and the D. melanogaster copia-like element 17.6 showed that Ty was most similar to copia, sharing amino acid sequence homology and organizational features not found in the other genetic elements.
Some of the signals for 3'-end formation in transcription of the Saccharomyces cerevisiae Ty-D15 element are immediately downstream of the initiation site
Fragments from the Ty-D15 element of Saccharomyces cerevisiae were assayed for the ability to direct 3'-end formation for RNA initiated by the GAL1 promoter and surprisingly, the delta region downstream of the initiation site for Ty RNA comprised part of this major site and terminated more than 50% of the transcripts that read into it.
Control of yeast gene expression by transposable elements.
The Ty (transposon yeast) elements of Saccharomyces cerevisiae comprise a family of dispersed, repeated DNA sequences that can be divided into two classes known as Ty1 and Ty2; these classes differ from each other by two large blocks of heterologous sequence.
Yeast retrotransposon Ty4: the majority of the rare transcripts lack a U3-R sequence.
It is concluded that Ty4 transcription is repressed by a negative regulating element residing within the LTR, whereas positive cis-acting elements, like those that have been found to mediate expression of Ty1/2 and Ty3, are absent from Ty4.
Ty RNA levels determine the spectrum of retrotransposition events that activate gene expression in Saccharomyces cerevisiae
This work characterized 206 spontaneous Ty transpositions that activate the promoterless gene his3Δ4, and found that chromosomal Ty transcripts are even more abundant than previously estimated and comprise about 1% of total cellular RNA.
A transposon-like element in human DNA
It is demonstrated that a repetitive human sequence has many of the diagnostic features of transposable elements, and members of this transposon-like family are found in extrachromosomal circular DNA molecules.
Bidirectional transcription from a solo long terminal repeat of the retrotransposon TED: symmetrical RNA start sites
A single copy of the retrotransposon TED was found integrated within the DNA genome of the insect baculovirus, Autographa californica nuclear polyhedrosis virus, and examined the effect of this solo TED LTR on the local pattern of viral transcription.