RNA extraction from polysaccharide-based cell-laden hydrogel scaffolds.

  title={RNA extraction from polysaccharide-based cell-laden hydrogel scaffolds.},
  author={Chunming Wang and Jinghua Hao and Feng Zhang and Kai Ming Su and Dong-An Wang},
  journal={Analytical biochemistry},
  volume={380 2},
An effective method for obtaining high-quality RNA from polysaccharide-rich hydrogels would be of great interest to biomaterialists and tissue engineers. Based on the similarities between polysaccharide-based hydrogels and plant tissues, we used a plant-specific RNA extraction kit to extract RNA from mammalian cells encapsulated in hydrogel scaffolds. The results indicate that this method can be reliably used in isolating high-purity RNA from polysaccharide hydrogels. 

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