RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties.

@article{Schiebel1993RNAdirectedRP,
  title={RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties.},
  author={W Schiebel and B. Haas and Snezan Marinkoviç and A Klanner and Heinz Ludwig S{\"a}nger},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 16},
  pages={
          11858-67
        }
}
The catalytic properties of electrophoretically homogeneous RNA-directed RNA polymerase (RdRP, EC 2.7.7.48) from tomato leaf tissue were studied with the aid of oligonucleotides of defined sequence. It was found that RdRP catalyzes in vitro the transcription of short single-stranded RNA and DNA molecules into precisely complementary RNA copies up to the full length of these templates. The transcription of RNA- and DNA-oligonucleotide templates was equally effective. Differences in transcription… CONTINUE READING
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