RNA-catalysed RNA polymerization using nucleoside triphosphates

@article{Ekland1996RNAcatalysedRP,
  title={RNA-catalysed RNA polymerization using nucleoside triphosphates},
  author={Eric H. Ekland and David P. Bartel},
  journal={Nature},
  year={1996},
  volume={382},
  pages={373-376}
}
THE hypothesis that certain RNA molecules may be able to catalyse RNA replication is central to current theories of the early evolution of life1–6. In support of this idea, we describe here an RNA that synthesizes RNA using the same reaction as that employed by protein enzymes that catalyse RNA polymerization. In the presence of the appropriate template RNA and nucleoside triphosphates, the ribozyme extends an RNA primer by successive addition of up to six mononucleotides. The added nucleotides… 

RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension

An RNA molecule is described that catalyzes the type of polymerization needed for RNA replication, which uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides.

Creation and evolution of new ribozymes.

An RNA is generated that synthesizes RNA using the same reaction as that employed by protein enzymes that catalyze RNA polymerization, and shows significant template fidelity.

Ribozyme-Catalyzed Transcription of an Active Ribozyme

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A DNA enzyme that mimics the first step of RNA splicing

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RNA Synthesis by in Vitro Selected Ribozymes for Recreating an RNA World

This review focuses on three types of ribozymes that could have been involved in the synthesis of RNA, the core activity in the self-replication of RNA world organisms.

Polymerase ribozyme efficiency increased by G/T-rich DNA oligonucleotides.

The results indicate that these DNA sequences function by establishing many weak and nonspecific base-pairing interactions to the single-stranded portion of the template, which could have had important functions in an RNA world.
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