RNA-binding properties of Dendrolimus punctatus tetravirus p17 protein.

  title={RNA-binding properties of Dendrolimus punctatus tetravirus p17 protein.},
  author={Liang Zhou and Yangli Zheng and Hong Jiang and Weidong Zhou and Meijuan Lin and Yajuan Han and Xu Cao and Jiamin Zhang and Yuanyang Hu},
  journal={Virus research},
  volume={138 1-2},
The RNA-binding properties of the p17 protein of Dendrolimus punctatus tetravirus were analysed. We have demonstrated that p17 protein, a nonstructural protein with a potentially important role in viral replication, is a RNA-binding protein, which has not been previously described for any member of the family Tetraviridae. P17 protein was expressed in Escherichia coli and was used in UV cross-linking analysis, using a digoxigenin-UTP-labeled RNA probe and chemical cross-linking analysis. The… CONTINUE READING